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Journal archive > 2009 > N 3 May-June

PROTEOLYTIC ACTIVITY OF IgG OF BLOOD SERUM OF PATIENTS
WITH SYSTEMIC LUPUS ERYTHEMATOSIS

Yu. Ya. Kit1, I. B. Magoryvska1, A. M. Gavrylyuk2, B. B. Chop’yak2, R. O. Bilyy1, R. S. Stoika1

1Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
2Danylo Halytsky Lviv Medical University, Ukraine;
e-mail: kit@сellbiol.lviv.ua

Proteolytic activity of blood serum IgGs of 10 patients with systemic lupus erythematosis (SLE) was studied in comparison with such activity in 10 clinically healthy donors. Antibodies were precipitated from blood serum by  saturation with 50% (NH4)2SO4 and IgG was isolated by the affinity chromatography on protein G-sepharose column. Histone H1 and core histones from the calf thymus, bovine myelin basic protein (MBP), lysozyme of chicken egg and bovine serum albumin (BSA) were used as substrates for proteolytic action. It was found that 4 of 10 preparations of IgGs possess an ability to hydrolyze both histone H1 and MBP. These antibodies practically did not cleave lysozyme of the chicken egg and BSA. Gel-filtration of antibodies under acidic condition and following examination of proteolytic activity of chromatographic fractions showed that histone H1 and MBP-hydrolyzing activity is attributable to IgG-antibodies. Thus, the presence of catalytically active antibodies (protabzymes) in the blood serum of patients with SLE has been demonstrated. Their origination and biological role are discussed.

Key words: systemic lupus erythematosis, serum, proteolytic activity, antibodies, protabzymes.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal