Journal archive > 2009 > N 2 March-April
S. I. Romanyuk, D.?V.?Kolibo, S. V. Komisarenko
The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diphtheriae. For this purpose we have constructed the immune library of murine immunoglobulin genes. A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 107 to 109 М-1.
Key words: scFv, single chain antibodies, library of immunoglobulin genes, phage display, diphtheria toxin.
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