Journal archive > 2009 > N 1 January-February

A PORTABLE SENSOR SYSTEM FOR PHENOLS DETECTION BASED
ON MOLECULARLY-IMPRINTED POLYMERS WITH CATALYTIC PROPERTIES

Т. А. Sergeyeva1, O. А. Slinchenko2, O.?O.?Brovko2, S. А. Piletsky3, G. V. Elska1

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Macromolecular Chemistry, National Academy of Sciences of Ukraine, Kyiv; 3Cranfield University, Great Britain;
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Portable devices for the express control of the phenols content in water were developed. Polymers mimicking active site of the enzyme tyrosinase, synthesized using the method of molecular imprinting, were obtained by co-polymerization of the complex Сu (II)-catechol-urocanic acid ethyl ester with ethyleneglycoldimethacrylate. Concentration of phenols in the analyzed samples was detected using universal portable device oxyme­ter with the oxygen electrode in a close contact with catalytic molecularly-imprinted polymer as a transducer. The detection limit of phenols detection using the developed sensor system based on polymers-biomimics with the optimized composition comprised 0.078 mM, while the linear range of the sensor comprised 0.078-5 mM. The working characteristics of the portable sensor devices were investigated. The possibility of repeated use of the catalytic polymers-biomimics as selective elements of the developed portable sensor systems was demonstrated. Storage stability of sensor systems at room temperature was 12 months (90%). As compared to traditional methods of phenols detection the developed sensor system is characterized by operation simplicity, compactness, and low cost.

Key words: phenols, polymers-biomimics, polymer catalysts, molecularly-imprinted polymers, sensor, tyrosinase, environmental monitoring.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal