title en

Journal archive > 2008 > N 6 November-December

OPTIMIZATION OF CONDITIONS FOR?IMMOBILIZATION OF ?-AMYLASE
FROM Bacillus sp. BKL20 IN?Ca2+-ALGINATE BEADS

O. I. Kubrak, V. I. Lushchak

Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;
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The method of ?-amylase from Bacillus sp. immobilization by an entrapment in Са2+-alginate beads has been performed in the paper. Maximum content of immobilized ?-amylase was observed at neutral pH values during immobilization. The approximately equal quantities of ?-amylase were entrapped into Са2+-alginate beads, that was confirmed by the similar total activity (~ 0.05 U) and declining specific activity (from 12 to 1 U/mg protein) of immobilized ?-amylase after the addition of different amounts of enzyme preparation (from 20 to 160 mg of protein) to 1 ml of 2% alginate solution. Alginate concentration affected the activity of immobilized enzyme, maximum ?-amylase activity was observed at low concentrations of the carrier (0.15–0.25%), used to prepare beads. ?-Amylase, entrapped in Са2+-alginate beads, was successfully tested during five hydrolysis cycles.

Key words: ?-amylase, Bacillus sp., immobilization, Са2+-alginate beads.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal