OBTAINING AND CHARACTERISTICS OF NATIONAL PREPARATION INTERFERON ?-2b WITH PROLONGED EFFECT (I.O. Pokholenko, L.V. Porubleva, I.Ya.?Dubey, А.V. Rebriev, L.P.?Sutugina, T.Yu. Gromovoy, V.O.?Pokrovskiy, M.Yu.?Obolenskaya, S.I.?Chernykh)

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Journal archive > 2008 > N 6 November-December

OBTAINING AND CHARACTERISTICS OF NATIONAL PREPARATION

INTERFERON ?-2b WITH PROLONGED EFFECT

I. O. Pokholenko¹, L. V. Porubleva¹, I.?Ya.?Dubey¹, А. V. Rebriev², L.?P.?Sutugina³,

T. Yu. Gromovoy⁴, V.?O.?Pokrovskiy⁴, M.?Yu.?Obolenskaya¹, S.?I.?Chernykh^1,3

¹Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;

²Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

³SPC «Interpharmbiotek», Kyiv;

⁴Chuyko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv;

е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Pegylated interferon ?-2b (PEG – IFN ?-2b) is a domestic preparation of a modified recombinant interferon ?-2b with prolonged effect. The preparation was obtained by N-terminal pegylation of IFN ?-2b with polyethylene glycol (PEG). This paper presents the method of PEG IFN ?-2b synthesis and characteristics of the obtained product. PAAG electrophoresis, Western blot analysis and MALDI-TOF mass-spectrometry confirm that the preparation is an N-terminal pegylated IFN ?-2b that contains no more than 10% of dipegylated IFN ?-2b. The comparison of PEG – IFN ?-2b with its foreign analogue has revealed the similarity of their biological activity and pharmacokinetic parameters.

Key words: interferon ?-2b, pegylation, MALDI-TOF mass-spectrometry, biological activity, pharmacokinetics.

The original article in Russian is available for download in PDF format.