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Journal archive > 2008 > N 6 November-December

OBTAINING AND CHARACTERISTICS OF NATIONAL PREPARATION
INTERFERON ?-2b WITH PROLONGED EFFECT
 
I. O. Pokholenko1, L. V. Porubleva1, I.?Ya.?Dubey1, А. V. Rebriev2, L.?P.?Sutugina3,

T. Yu. Gromovoy4, V.?O.?Pokrovskiy4, M.?Yu.?Obolenskaya1, S.?I.?Chernykh1,3

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3SPC «Interpharmbiotek», Kyiv;
4Chuyko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv;

Pegylated interferon ?-2b (PEG – IFN ?-2b) is a domestic preparation of a modified recombinant interferon ?-2b with prolonged effect. The preparation was obtained by N-terminal pegylation of IFN ?-2b with polyethylene glycol (PEG). This paper presents the method of PEG IFN ?-2b synthesis and characteristics of the obtained product. PAAG electrophoresis, Western blot analysis and MALDI-TOF mass-spectro­metry confirm that the preparation is an N-terminal pegylated IFN ?-2b that contains no more than 10% of dipegylated IFN ?-2b. The comparison of PEG – IFN ?-2b with its foreign analogue has revealed the similari­ty of their biological activity and pharmacokinetic parameters.

Key words: interferon ?-2b, pegylation, MALDI-TOF mass-spectrometry, biological activi­ty, pharmacokinetics.

The original article in Russian is available for download in PDF format.

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