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Journal archive > 2008 > N 6 November-December

INVESTIGATIONS OF FUNCTIONAL GROUPS
OF Penicillium commune 266 ?-L-RHAMNOSIDASES

O. N. Rzaeva, N. V. Borzova, L.D.Varbanets

Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
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On the basis of the data of metal ions influen­ce it was established that Penicillium commune 266 ?-L-rhamnosidases are dependent of metals. The cations of Cu2+, Mn2+, Ag+, Hg2+ essentially influen­ced the activity decreasing its up to 45–55%. Ba2+ and Mn2+ ions completely inhibited ?-L-rhamnosidase 2 activity in concentration of 10-3?М. The role of carboxylic group and histidine imidazole group in hydrolysis of rhamnoglycosides have been estimated by the Diхon, Lineweaver-Burke and photooxidation methods. It was also shown that sulfhydrilic groups which do not take part in catalysis, but are necessary for displaying the enzymatic activity are located at a certain distance from P.?commune 266 ?-L-rhamnosidase active centre.

Key words: ?-L-rhamnosidase, Penicillium commune, functional groups, active centre.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal