title en

Journal archive > 2008 > N 6 November-December

ACTIVITY OF GLUTATHIONE-DEPENDENT ANTIOXIDANT SYSTEM OF RAT LIVER
AND BLOOD DEPENDING ON ?-IRRADIATION AND DIET

Yu. V. Nikitchenko, V. I. Padalko, V.N.Tkachenko , A. A. Sheremet, V.V.Tovstiak

Karazin Kharkov National University, Ukraine;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The role of the glutathione-dependent antioxidant system in the prooxidant-antioxidant balan­ce support was studied in the experiments with Wistar male rat under single ?-irradiation (8 Gr dose), fed with unbalanced (as to animal proteins and antioxidant vitamins) diet and with addition of Aronia melanocarpa. Single ?-irradiation of animals led to the decrease of selenium-dependent glutathione-peroxidase activity in the blood plasma and glutathio­ne-S-transferase activity decrease in rat liver mitochondria. Animals which received the unbalanced food allowance under irradiation showed more expressed change of glutathione-dependent antioxidant enzymes activity, namely – proved dec­rease of glutathione-peroxidase and glutathione-S-transferase activity in liver microsomes and less expressed activation of selenium-dependent glutathione-peroxidase activity in the postmitochondrial fraction of laboratory animals liver. Introduction of the A. melanocarpa food supplement in the unbalanced diet of the laboratory animals which in vitro demonstrated expressed antioxidant and antiradical activity had no effect upon glutathione-peroxidase activity in the investigated tissues. Obtained data concerning significant decrease of the activity of glutathione-dependent antioxidant system and, particularly, of the selenium-dependent glutathione-peroxidase activity under the unbalanced diet condition may be useful in maintenance of prooxidant-antioxidant balance in the tissues of irradiated animals. Allowing for the above stated it is advisable to seek for new food additives which increase activity of the endogenous glutathione-dependent antioxidant enzymes for human tolerance improvement, especially under the unbalanced food allowance condition.

Key words: glutathione-dependent antioxidant system, unbalanced diet, ?-irradiation, Aronia melanocarpa.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal