DIAZOXIDE-INDUCED MITOCHONDRIAL SWELLING IN THE RAT MYOMETRIUM AS?A?CONSEQUENCE OF THE ACTIVATION OF THE MITOCHONDRIAL ATP-SENSITIVE K+-CHANNEL (О.B. Vadzuk, S.О. Kosterin)

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Journal archive > 2008 > N 5 September-October

DIAZOXIDE-INDUCED MITOCHONDRIAL SWELLING IN THE RAT MYOMETRIUM AS?A?CONSEQUENCE

OF THE ACTIVATION OF THE MITOCHONDRIAL ATP-SENSITIVE K⁺-CHANNEL

О. B. Vadzuk, S. О. Kosterin

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;

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In this paper we postulate, that mitochondria isolated from the rat myometrium undergo swelling in isoosmotic medium, which contains K⁺ (125?mM). This swelling was blocked by ATP (200?µM), but only when MgCl₂ (1 mM) was present and observed when oligomycin, the inhibitor of F_oF₁-ATPase, was added to the incubation medium. Diazoxide (50 µM), activator of the mitochondrial ATP-sensitive potassium channel, removed ATP-induced blockade of swelling. Our results may prompt the presence of K⁺ transporter on the inner mitochondrial membrane, which possesses the features of the mitochondrial ATP-dependent potassium channel described earlier in mitochondria of the heart, liver, brain, retina, blood vessels and kidneys.

Key words: mitochondrial ATP-sensitive potassium channel, diazoxide, mitochondria, mitochondrial swelling, myometrium.

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