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Journal archive > 2008 > N 5 September-October

DIAZOXIDE-INDUCED MITOCHONDRIAL SWELLING IN THE RAT MYOMETRIUM AS?A?CONSEQUENCE
OF THE ACTIVATION OF THE MITOCHONDRIAL ATP-SENSITIVE K+-CHANNEL

О. B. Vadzuk, S. О. Kosterin

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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In this paper we postulate, that mitochondria isolated from the rat myometrium undergo swelling in isoosmotic medium, which contains K+ (125?mM). This swelling was blocked by ATP (200?µM), but only when MgCl2 (1 mM) was present and observed when oligomycin, the inhibitor of FoF1-ATPase, was added to the incubation medium. Diazoxide (50 µM), activator of the mitochondrial ATP-sensitive potassium channel, removed ATP-induced blockade of swelling. Our results may prompt the presence of K+ transporter on the inner mitochondrial membrane, which possesses the features of the mitochondrial ATP-dependent potassium channel described earlier in mitochondria of the heart, liver, brain, retina, blood vessels and kidneys.

Key words: mitochondrial ATP-sensitive potassium channel, diazoxide, mitochondria, mitochondrial swelling, myometrium.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal