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Journal archive > 2008 > N 4 July-August

INTRAMOLECULAR DOUBLE-STRANDED HELICES OF POLYADENYLIC ACID
AND THEIR POSSIBLE BIOLOGICAL SIGNIFICANCE

M. I. Zarudnaya, A. V. Stepanyugin, A. L. Potyahaylo, D. М. Hovorun

Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
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Under protonation of the single-stranded polyadenylic acid so called «frozen» form arises simultaneously with formation of two double-stranded­ forms. It has been shown that a portion of the polyadenylic acid «frozen» form increases with the size of poly(A) and ionic strength of solution, i. e. with an increase of polymer chain flexibility. These findings may be considered as evidences in favor of our earlier supposition on poly(A) ability to fold with formation of intramolecular double-stranded helices. Ten years ago we also proposed a hypothesis that such double-stranded helices can be formed in poly(A) tracts of cellular RNAs and they can participate in several biological processes. An analysis of literature data published later evidences for the hypothesis adequacy and shows that it still remains urgent.

Key words: poly(A), electrophoresis, «frozen» form, intramolecular double-stranded helices, polyadenylation termination, autoregulation of cytoplasmatic poly(A) binding protein synthesis, mRNA stabilization.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal