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Journal archive > 2008 > N 4 July-August

CYTOCHROME P450 2E1 EXPRESSION IN MICE LIVER UNDER EXPOSURE
OF CONTINUOUS AND ACUTE ?-RADIATION

O. V. Maksymchuk1, L. K. Bezdrobna2, L. L. Sidorik1, O. K. Kiseleva1, M. O. Chaschyn1

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Nuclear Research, National Academy of Sciences of Ukraine, Kyiv;
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The effect of continuous low-intensity and acute high-intensity ionizing radiation in low and high doses on expression of cytochrome P450 2E1 (1.14.14.1) in mice liver was studied. It was shown, that changes of Cyp2e1 amount depended on the dose and intensity of ionizing radiation. Low doses of continuous ?-radiation caused reliable decrease of Cyp2e1 expression on protein mRNA levels. Low doses of acute ?-radiation lead to an increase of Cyp2e1 amount, while high doses – to a corresponding decrease. We showed, that low and high doses of acute ?-radiation caused a significant decrease of Cyp2e1 mRNA level. We suppose that the detected changes of Cyp2e1 expression are associated with peroxidation process and development of oxidative stress.

Key words: Cytochrome P450 2E1, acute and continuous ?-radiation, Cyp2e1 mRNA and protein expression, oxidative stress.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal