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Journal archive > 2008 > N 3 May-June  

SENSOR SYSTEM BASED ON?MOLECULARLY-IMPRINTED POLYMER MEMBRANES
FOR?THE?SELECTIVE RECOGNITION OF AFLATOXIN B1

Т. А. Sergeyeva1, О. V. Piletska2, L. A. Goncharova3, О. О. Brovko3, S. А. Piletsky2, G. V. El’ska1

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Cranfield University at Silsoe, United Kingdom;
3Institute of Macromolecular Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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Synthetic analogues of biological receptors able to selective recognition of aflatoxin B1 were obtained using the method of molecular imprinting. They were used as a basis for the fluorescent test-system for the selective revealing of aflatoxin B1. The composition of molecularly-imprinted polymers able to individual recognition of aflatoxins was optimized using the method of computational modeling. This method provides selection of functional monomers able to interact with different parts of the molecule of mycotoxins affiliated to aflatoxins due to non-covalent interactions. The synthetic receptors able to individual recognition of aflatoxin B1 from the mixture of its close structural analogues (aflatoxins B2 and G2) were obtained. Acrylamide and 2-acrylamido-2-methyl-1-propanesulfonic acid providing high binding energies (- 19,06 – -27,11 kcal/M) with aflatoxin B1 were used as functional monomers for the synthesis of aflatoxin-B1-selective receptors. Molecularly-imprinted polymers with the optimized composition were obtained in a form of porous polymeric membranes based on interpenetrating polymer networks. Ethyl-2-oxocyclopentanecarboxylate (structural analogue of aflatoxin B1) was used during the synthesis of moleculalrly-imprinted polymer membranes as a template molecule. It was shown that ethyl-2-oxocyclopentanecarboxylate-imprinted po-lymer membranes, synthesized using acrylamide as a functional monomer, were characterized by sufficient mechanical stability and high adsorbtion capability towards aflatoxin B1. The molecularly-imprinted polymer membranes were characterized by the pronounced imprinting effect as well as by insignificant adsorbtion of aflatoxins B2 and G2. Therefore, the synthetic analogues of bioreceptors able to individual recognition of aflatoxin B1 were obtained and used as a basis for the optical sensor system for aflatoxin B1 detection in a concentration range 1–500 ng/ml.

Key words: aflatoxin B1, molecular imprinting, molecularly-imprinted polymer, solid-phase extraction, sensor.

The original article in Ukrainian is available for download in PDF format.

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