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Journal archive > 2008 > N 3 May-June  

ROLE OF ?-TOCOPHEROL IN OXIDATIVE STRESS OF RAT THYMOCYTES
INDUCED BY HYDROGEN PEROXIDE AND?MENADIONE

G. V. Petrova, G. V. Donchenko

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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The paper deals with studying the effect of ?-tocopherol and its analogues (?-tocopheryl acetate and ?-tocopheryl quinine) showing no antioxidant properties on rat thymocytes survival and intracellular content of reactive oxygen species (ROS) at H2O2 and menadione-induced oxidative stress. It is established, that the ability of ? tocopherol to inhibit the thymocytes destruction induced by the development of oxidative stress is insignificant. It does not depend on the presence of free OH-group in the structure of ?-tocopherol molecule responsible for development of antioxidant properties, and does not correlate with its influence on intracellular ROS content. The efficiency of the glutathione synthesis predecessor N-acetyl-L-cysteine in the given model testifies to the involving of other systems of antioxidative protection in these processes. The obtained data allow to conclude, that ?-tocopherol does not play the main role in the protection of cells against ROS damaging action  that calls into question its ability to prevent the oxidative stress.

Key words: ?-tocopherol, ?-tocopheryl acetate, ?-tocopheryl quinone, menadione, hydrogen peroxide, oxidative stress, reactive oxygen species, N-acetyl-L-cysteine.

The original article in Russian is available for download in PDF format.

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