title en

Journal archive > 2008 > N 3 May-June  

INFLUENCE OF DIFFERENT SOURCES OF CARBON AND NITROGEN ON THE BIOSYNTHESIS
OF PROTEOLYTIC COMPLEXES BY Вacillus circulans 693, Вacillus sp. 27
AND Yarrowia lipolytica 2061 STRAINS

А. S. Shubchinskaya, O. V. Matselyukh, L. D. Varbanets

Institute of Microbiology and Virology, National Academy of Sciense of Ukraine, Kyiv;
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The influence of different factors on the biosynthesis of extracellular proteolytic complexes by strains-producers Вacillus circulans 693, Вacillus sp. 27 and Yarrowia lipolytica 2061 at submerged cultivation has been investigated. It has been shown that ammonium hydrocarbonate and gelatin with glucose were optimum carbon and nitrogen sources for synthesis of proteolytic activity of B. circulans strain 693, gelatin with arabinose – for Bacillus sp. 27, gelatin and glycine with sorbose – for Y.?lipolytica 2061. It has been established that the cultivation of producers on optimal sources of carbon and nitrogen increased protease activity of cultural liquid of B.?circulans 693 3.8 times, Bacillus sp. 27?– 2.7 times, Y. lipolytica 2061 – 3.4 times. It has been found that the usage of different protein substrates in cultural medium permitted to obtain the proteolytic enzymes with various specificity with respect to globular and fibrillar proteins.

Key words: Вacillus circulans, Вacillus sp., Yarrowia lipolytica, extracellular proteolytic complexes, cultivation conditions.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal