title en

Journal archive > 2008 > N 2 March-April
HEME OXYGENASE ACTIVITY IN?THE TISSUES OF THE VESSELS AND HEART OF RATS
UNDER CO ADMINISTRATION OF NO SYNTHASES AND HEMIN CHLORIDE

P. A. Kaliman, V. P. Filimonenko, I. V. Nikitchenko

Karazin Kharkov National University, Ukraine;
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 The administration of hemin chloride in a dose of 1.5 mg/100 g of the body weight was found to cause accumulation of the total heme and TBA-reactive products in the rat blood serum and vessels. Pretreatment by N?-nitro-L-arginine (0.5?h before hemin chloride administration) did not affect the dynamics of the total heme and TBA-reacting products accumulation. The increase of heme oxygenase activity was observed in the vessels after hemin chloride administration. This effect was strengthened by N?-nitro-L-arginine pretreatment. The changes of heme oxygenase activity and the total heme level in heart were not observed at any periods studied. The increase of the TBA-reactive products level in the heart after exogenous hemin injection was accompanied by an increase of nitrites content and blocked by pretreatment of NOS inhibitor. The N?-nitro-L-arginine alone caused the accumulation of the total heme, TBA-reacting products and the increase of heme oxygenase activity in the vessels. The role of heme and NO in regulation of the heme oxygenase activity is discussed.

Key words: heme oxygenase, heme, nitric oxide, hemin chloride, N?-nitro-L-arginine.

The original article in Russian is available for download in PDF format.

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