title en

Journal archive > 2008 > N 2 March-April 

EFFECT OF 2,4-DINITROPHENOL ON THE RAT LIVER RESPIRATORY ACTIVITY
AND ATP CONTENT AFTER HYPOTHERMIC STORAGE AND FOLLOWING REPERFUSION

D. V. Cherkashina, O. M. Tkachova, O. Yu. Somov, O. A. Semenchenko, O. S. Lebedinsky, O. Yu. Petrenko

Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkov;

The influence of oxidative phosphorylation uncoupler 2,4-dinitrophenol (DNP) presence in preserving solution on the rat liver respiratory activity and ATP content after 18 h of hypothermic storage (HS) and following normothermic reperfusion (NR) was investigated. DNP presence on the HS stage led to decrease of ATP level as compared with the control. After DNP removal during­ NR the gradual recovery of oxidative phosphory­lation coupling occurred. This fact resulted in improvement of mitochondrial functional state (V4 respiration rate decrease, respiratory control and ATP level increase).

Key words: 2,4-dinitrophenol, liver hypothermic storage, normothermic reperfusion, respiratory activity, oxidative phosphorylation, liver ATP level.

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