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Journal archive > 2008 > N 2 March-April

CHANGE IN GLUTATHIONE CONTENT IN RAT THYMOCYTES UNDER APOPTOSIS
INDUCED BY Н2О2 OR X IRRADIATION

T. V. Koval, O. О. Nazarova, О. P. Мathyshevska

Taras Shevchenko Kyiv National University, Ukraine;
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Glutathione (GSH) content as well as GSH-peroxidase and GSH-reductase activity in isolated rat thymocytes X-irradiated in a dose of 4.5 Gy or treated with 0.1 mM H2O2 were studied in a period preceding the appearance of apoptosis morphological symptoms. The early adaptive response of thymocytes to radiation – increase of both GSH content and glutathione peroxidase and glutathione reductase activity was revealed. On the contrary the rapid fall of GSH level in H2O2-treated thymocytes was observed simultaneousely with glutathione reductase inhibition and enhanced GSH consumption by glutathione peroxidase, this disbalance of GSH-dependent antioxidant system probably facilitates mitochondrial way of apoptosis.

Key words: apoptosis, glutathione, GSH-peroxidase, GSH-reductase, thymocytes, hydrogen peroxide, X-irradiation.

The original article in Ukrainian is available for download in PDF format.

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