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Journal archive > 2008 > N 2 March-April 


O. A. Shkrabak1, О. I.  Kalchenko2, R. V. Rodik2, T. O. Veklich1, V. I. Kalchenko2, S. O. Kosterin1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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It was shown that сalix[4]arene bis-aminomethylphosphonic acid С-107 can hydrolyze ATP. The kinetic curve of the ATP hydrolysis induced by сalixarene С-107 was nonhyperbolic and had a tendency to plateau (in the course of time) observing from 45-60 minutes of the incubation period when the reaction practically came to the end. The empirical kinetic characteristics of this reaction were calculated. The velocity of calixarene-dependent hydrolysis of АТР exceeds the velocity of spontaneous hydrolysis of АТР at least 14–15 times. The «Host–Guest» complexation of the сalixarene С-107 with adenosinetriphosphate in acetonitrile/water (47/53 v/v) solution was investigated by the reversed-phase high performance liquid chromatography. The dissociation constants of the 1 : 1 «Host–Guest» complexes of ATP-Guest with the calixarene-Host when using­ two columns Zorbax CN and LiChrosorb RP 18 within 197–231 µМ were determined from the capacity factor of the Guest and concentration of the calixarene-Host in the mobile phase. The electrostatic, ion-dipole, dipole-dipole C-H-? and other weak interactions in the «Host–Guest» complexes were discussed. Obtained data can be a basis for designing the synthetical ATP-hydrolyzing catalysts and also for subsequent investigation of both enzymatic and nonenzymatic ATP hydrolysis reaction,processes of ATP-dependent Ca2+-transporting in subcellular membrane structures.

Key words: hydrolysis of ATP, calixarenes, empirical kinetic analysis, reversed-phase high performance liquid chromatography, molecular modeling.

The original article in Ukrainian is available for download in PDF format.

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