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Journal archive > 2008 > N 2 March-April 

CALIXARENE-DEPENDENT HYDROLYSIS OF АТР. І. KINETICS AND COMPLEXATION
OF THE СALIXARENE С-107 WITH ADENOSINETRIPHOSPHATE

O. A. Shkrabak1, О. I.  Kalchenko2, R. V. Rodik2, T. O. Veklich1, V. I. Kalchenko2, S. O. Kosterin1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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It was shown that сalix[4]arene bis-aminomethylphosphonic acid С-107 can hydrolyze ATP. The kinetic curve of the ATP hydrolysis induced by сalixarene С-107 was nonhyperbolic and had a tendency to plateau (in the course of time) observing from 45-60 minutes of the incubation period when the reaction practically came to the end. The empirical kinetic characteristics of this reaction were calculated. The velocity of calixarene-dependent hydrolysis of АТР exceeds the velocity of spontaneous hydrolysis of АТР at least 14–15 times. The «Host–Guest» complexation of the сalixarene С-107 with adenosinetriphosphate in acetonitrile/water (47/53 v/v) solution was investigated by the reversed-phase high performance liquid chromatography. The dissociation constants of the 1 : 1 «Host–Guest» complexes of ATP-Guest with the calixarene-Host when using­ two columns Zorbax CN and LiChrosorb RP 18 within 197–231 µМ were determined from the capacity factor of the Guest and concentration of the calixarene-Host in the mobile phase. The electrostatic, ion-dipole, dipole-dipole C-H-? and other weak interactions in the «Host–Guest» complexes were discussed. Obtained data can be a basis for designing the synthetical ATP-hydrolyzing catalysts and also for subsequent investigation of both enzymatic and nonenzymatic ATP hydrolysis reaction,processes of ATP-dependent Ca2+-transporting in subcellular membrane structures.

Key words: hydrolysis of ATP, calixarenes, empirical kinetic analysis, reversed-phase high performance liquid chromatography, molecular modeling.

The original article in Ukrainian is available for download in PDF format.

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