Journal archive > 2008 > N 1 January-February

 INFLUENCE OF DIRECT ELECTRIC CURRENT ON HYDRODYNAMIC
DIAMETER OF HUMAN SERUM ALBUMIN

M. I. Korpan1, V. F. Gorchev2, I. S. Chekman3, V. M. Gun'co4, V. Fialka-Moser1

1Vienna Medical University, Austria;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
3Bogomolets National Medical University, Minisrty of Public Health, Kyiv, Ukraine;
4Chuyko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv;
е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The effect of the weak electric current (2?mA/cm2) on structural characteristics (hydro-dynamic diameter and molecular weight) of the human serum albumin (HSA) was studied using­ photon correlation spectroscopy (PCS). The average diameter of initial HSA globule is approximately 7 nm (66.8 kDa). After electric current treatment during 2–5 min the diameter of HSA monomer increases to 7.5 nm. The duration of electric current treatment being increased to 20?min the size of HSA monomers decreases to 6.4 nm. The behavi­our of HSA oligomers is close to that of monomers. Consequently, changes in the sizes of monomers and oligomers of HSA under the electric current treatment are caused by the change in the charge density stimulating change of tertiary structure of molecules and possible addition of ions from the buffer solution to them.

Key words: photon correlation spectroscopy, electric current, serum albumin.

The original article in Russian is available for download in PDF format.

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