Journal archive > 2008 > N 1 January-February
E. F. Gerych, A. A. Pentyuk
The feeding of rats with high-fat diet (a part of fats was 50% of energy value of ration against 20% in control) during 4 weeks increased the level of cytoсhrome Р-450 in the liver and enhanced aniline- and p-nitrophenol hydroxylase activity of СУР2Е1 as well as erythromycin N-demethylase activity of СУР3А; the activity of aldehyde dehydrogenase class 3, UDP-glucuronosyl transferase, glutathione-S-transferase and N-acеtyl transferase. At the same time, the moderate decrease of indomethacin-O-demethylase of СУР2С both phenolsulfotransferase activity were fixed. The changes of enzymatic activity correlated with activating of processes of gluconeogenesis, glycogenolysis and, especially, ketogenesis. A high-fat diet enhanced reactions of biotransformation of amidopyrine, acetanilide, toluene, sulfadimezine, were catalyzed with СУР2Е1, CYP3A and enzymes of conjugation, simultaneously it increased the hepatotoxicity of paracetamol.
Key words: high-fat diet, xenobiotics, cytoсhrome of Р-450, enzymes of conjugation, acetanilide, amidopyrine, toluene, paracetamol, toxicity.
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