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Journal archive > 2007 > N 6 November-December

THE N-STEAROYLETHANOLAMINE EFFECT ON THE NO-SYNTHASE WAY OF NITROGEN OXIDE
FORMATION AND?PHOSPHOLIPID COMPOSITION OF ERYTHROCYTE MEMBRANES IN RATS
WITH STREPTOZOTOCINE DIABETES

G. V. Kosiakova, N. M. Gulaya

Palladin Institute of Biochemistry, National Academy of Science of Ukraine, Kyiv;
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The influence of N-stearoylethanolamine (NSE) on the NO-synthase way of NO generation and phospholipids composition of erythrocyte membranes of rats with streptozotocine-induced diabetes has been studied. It has been shown that the activation of iNOS activity, cNOS activity inhibition and increase of the stable NO metabolites content takes place in the red blood cells (RBC) of diabetic rats. The alterations were also found in the RBC membrane phospholipid content: a decrease of phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, sphingomieline content and increase of phosphatidylethanolamine, phosphatidylcholine lysoforms level. The NSE suspension administration (50 mg/kg of body weight) to diabetic rats (3 months after the diabetes induction) resulted in iNOS activity inhibition, recove­ring of cNOS activity and normalization of NO stable metabolites level in RBC. The decrease of phospholipids lysoform levels, normalization of phosphatidylethanolamine, phosphatidylcholine content and increase of phosphatidylinositol level were found after NSE action.

Key words: N-stearoylethanolamine, nitric oxide, NO-synthase, membrane phospholipids, diabetes mellitus.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal