title en

Journal archive > 2007 > N 5 September-October  

OBTAINING OF RECOMBINANT scFv-ANTIBODIES AGAINST
DIPHTHERIA TOXIN USING PHAGE DISPLAY SYSTEM
O. S. Oliinyk1, A. A. Kaberniuk1, D. O. Burkaleva1, S. I. Romaniuk1, D. V. Kolibo1,
A. O. Shepelyakovskaya2, A. G. Laman2, S. V. Komisarenko1
1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;
2Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow

Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 · 107-1 was selected. scFv preserved activity on storage in the course of 8 months.

Key words: scFv, phage display, diphtheria toxin, recombinant proteins.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal