title en

Journal archive > 2007 > N 5 September-October  

INVESTIGATION OF MECHANISMS OF SYNAPTIC VESICLES FUSION
WITH ACCEPTOR MEMBRANES ON THE MODEL OF EXOCYTOSIS

G. P. Volynets, I. O. Trikash

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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Fusion of synaptic vesicles with various target membranes was investigated on the cell-free model system that reflects the final step of exocytosis. Plasma membranes, synaptic vesicles and liposomes were used as acceptor membranes. The process of membrane fusion was triggered by Ca2+. We have demonstrated that synaptic vesicles are prone to fuse with liposomes in buffer solution. This process was strongly dependent on ionic force of medium and phospholipid composition of liposomes. Cytosolic proteins of synaptosomes inhibi­ted the fusion of synaptic vesicles with liposomes, while these were required for the fusion of synaptic vesicles with native membrane structures. Trypsinolysis of acceptor membranes markedly inhibited the fusion response. It means protein components of target membrane are necessary for realization of the final step of exocytosis.

Key words: synaptic vesicles, membrane fusion, cell-free model system, fluorescent probe R18.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal