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Journal archive > 2007 > N 5 September-October  

AFLATOXIN-SELECTIVE MOLECULARLY-IMPRINTED POLYMER MEMBRANES BASED
ON ACRYLATE-POLYURETHANE SEMI-INTERPENETRATING POLYMER NETWORKS

Т. А. Sergeyeva1, О. V. Piletska2, О. О. Brovko3, L. A. Goncharova3, S. А. Piletsky2, G. V. El’ska1

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Cranfield University at Silsoe, Great Britain;
3Institute of Macromolecular Chemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Synthetic analogs of biological receptors able to group-selective recognition of aflatoxins were obtained using the combination of the technique of molecular imprinting with the method of computer modeling. The synthetic receptors were obtained in a form of thin and porous membranes based on semi-interpenetrating polymer networks. The selection of functional monomers able to non-covalent interactions with aflatoxins B1, B2, and G2 was based on the data of computer mode-ling. Allylamine, diethylaminoethylmethacrylate, and N,N?-methylenebisacrylamide, providing high binding energies with aflatoxins B1, B2, and G2 were selected as functional monomers for the formation of aflatoxin B1-imprinted polymer membranes. It was shown that aflatoxin-B1-imprinted polymeric membranes synthesized using N,N?-methylenebisacrylamide as a functional monomer were characterized with good physico-mechanical properties as well as good adsorbtion capability towards aflatoxin B1. Neglidgible levels of aflatoxin B1 adsorbtion on the surface of blank membranes were observed. High adsorbtion capability of the MIP membranes towards mycotoxins affiliated to the group of aflatoxins was demonstrated, while negligible adsorbtion of ochratoxin A was observed. Therefore, synthetic analogs of biological receptors able to group-selective recognition of aflatoxins in the range 1–1000 ppb were developed.

Key words: molecular imprinting, mole-cularly-imprinted polymer, solid-phase extraction, aflatoxin B1.

The original article in Ukrainian is available for download in PDF format.

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