title en

Journal archive > 2007 > N 5 September-October  

PARTICIPATION OF CYCLIC NUCLEOTIDES, PROTEIN KINASES A AND C IN REALIZATION
OF N-ACYLETHANOLAMINES ACTION IN HUMAN ADRENOCORTICAL CELLS

O. I. Kovzun1, N. I. Levchuk1, N. M. Gula2, A. S. Mikosha1

1Komissarenko Institute of Endocrinology & Metabolism, Academy of Medical Sciences of Ukraine, Kyiv;
2Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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The messenger mechanisms mediating N-acylethanolamines (NAE) regulatory signals in the adrenal cortex were studied. An analysis of the mechanisms of realization of NAE effects in the post-operation human adrenal cortex was carried out in vitro.  Influence of NAE mix on cAMP and cGMP level, protein kinase A and C activi­ty in sub-cellular fraction of adrenocorticocytes and homogenates of conditionally normal adrenal cortex tissues was investigated. It was shown, that N-acylethanolamines treatment resulted in a decrease of cAMP level in adrenocortical cells. cGMP level is not changed in these conditions. The rise of protein kinase C activity was obtained in the membrane fraction after N-acylethanolamines in vitro treatment (3.3 µg/ml). Activity of cAMP-dependent protein kinase A significantly decreased in cytosol fraction of adrenocorticocytes. It was concluded, that steroid genesis activation is determined by protein kinase C activation, inhibition is determined by cAMP-dependent messenger system.

Key words: N-acylethanolamines, adreno­corticocytes, сАМР, сGMP, protein kinase А, protein kinase С.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal