CALIXARENE C-91 STIMULATES Ca2+ ACCUMULATION IN MITOCHONDRIA
S. G. Shlykov1, L. G. Babich1, N. M. Slinchenko1 , R. V. Rodik2, V. I. Boyko2, V. I. Kalchenko2, S. O. Kosterin1
Calixarenes, owing to the ability to form supramolecular complexes with biologically important molecules and ions, can influence a course of biochemical processes and, accordingly, be considered as perspective molecular platforms for creation of physiologically active compounds. The work purpose is to study calixarene C-91 influence on systems of active Ca ions transport which are localized in subcellular membrane structures (mitochondria, sarcoplasmic reticulum, plasma membrane) of myometrial cells. It has been shown, that calixarene C-91 addition to incubation medium led to an increase in Ca2+ accumulation level in mitochondria. The maximal stimulating effect was 173% and it was observed at 100 µM concentration. It is suggested, that calixarene C-91 can enter mitochondria with the subsequent precipitation of Са ions in a matrix therefore calcium capacity increases, and as a consequence, higher Ca2+ accumulation in these structures is observed. In a wide range of concentration (1–100 µM) calixarene C 91 did not influence a level of Ca2+ accumulation in sarcoplasmic reticulum of myometrial cells. Titration of solubilized Са2+,Mg2+-АТРase by calixarene C-91 (0,1–100 µM) did not cause changes in its activity. Thus, calixarene C-91 increases Ca2+ accumulation level in mitochondria, but practically does not influence calcium pumps activity of a plasma membrane and sarcoplasmic reticulum of myometrial cells.
Key words: Ca ions, calixarene C-91, Mg2+-АТР-dependent Ca ions transport, mitochondria, sarcoplasmic reticulum, plasma membrane, myometrium.
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