G. V. Petrova
The development of hemolysis and methemoglobin formation under influence of vitamin?E derivatives was investigated on the in vitro model of rat red blood cells. It is established, that the shortening of lateral chains of ?-tocopherol (Т), ?-tocopheryl acetate (ТАc) and ?-tocopheryl quinone (ТQ) to 6 atoms of carbon (accordingly С6-Т, С6- ТАc, С6-ТQ) results in occurrence of hemolysis and prooxidative properties, which are not characteristic of derivatives with native length of isoprenoid side chains. Modifications of chroman ring of short-chain derivatives, in turn, bring the contribution to mechanisms of their action. So, the effect of С6-ТАc is caused, basically, physical destabilization of a plasmatic membrane, in С6-ТQ effect prevails prooxidative mechanism, while С6-Т equally shows membrane-destabilizing and prooxidative properties. Thus the derivatives Т with the modified structure chroman nucleus and variability of isoprenoid side chains length show absolutely various properties and in each concrete case should be considered as independent biologically active substances irrespective of T properties.
Key words: ?-tocopherol short-chain derivatives, rat red blood cells, hemolysis, methemoglobin.
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