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Journal archive > 2007 > N 4 July-August 

IN?MEMBRANE OF Bacillus sp. B4253 AND Bacillus sp. B4851

G. V. Danylovych1, T. G. Gruzina2, Z. R. Ulberg2, S. O. Kosterin1

1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;
2Ovcharenko Institute of Biocolloidal Chemistry, National Academy of Sciences of Ukraine, Kyiv;

Accumulation of gold in cells of Bacillus sр. B4253 can be directly or indirectly connected with activity of bacteria plasma membrane basal Mg2+-АТР­аse. Therefore this work deals with a comparative analysis of kinetic properties of plasma membrane basal аzide-resistant Mg2+-dependent АТР-hydrolase activity of B. sр. B4253 and B. sр. B4851 capable to gold accumulation and not capable to this process, accordingly. It is shown, that by a number of kinetic parameters – specific fermentative activity, initial speed of reaction of hydrolysis АТР (V0), Mixaelis constant (Km), the maximal initial speed by Mg2+ (VMg) and by АТР (VАТР), optimum concentration of АТР ([ATP]opt), рНmax, sensitivity to action of the thapsigargine and eosine Y?– bacteria membranes basal Mg2+-АТРаse activity accumulating gold, and the bacteria not capable to this process, are identical. But by some parameters they differ: Mg2+-АТРаse activity of membranes of the bacteria which do not accumulate gold, has three times greater affinity for Mg ions and smaller value [Mg]opt. The inhibition effect of ionic gold (10-4–3·10-4?M) is shown on аzide-sensitive (Н+-АТРase) and аzide-resistant (Mg2+-АТРаse) components Mg2+-dependent АТР-hydrolase activity in fraction of plasma membranes of microorganisms Bacillus accumulating gold, and not capable to this process. Colloid gold (0.0002–4 µg/ml) stimulates activity of Н+-АТРаse and Mg2+-АТРаse in a membrane of the bacteria accumulating gold 1.5–2 times, and does not influence activity of АТРаses of a membrane of the bacteria which do not accumulate gold.

Key words: ionic and colloid gold, Н+-АТРаse, Mg2+-АТРаse, Bacillus sр. B4253, B. sр. B4851.

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