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Journal archive > 2007 > N 4 July-August 

DETECTION OF EARLY APOPTOTIC ALTERATIONS IN CELLS IN VITRO
BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

А. А. Philchenkov, V. M. Mikhailenko, М. P. Zavelevich

R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology,
National Academy of Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The technique of proton magnetic resonance spectroscopy (1H MRS) was used as the sensitive express method for early specific detection of the apoptotic cells. The technique allows recognition of the changes in signal intensities corresponding to methylene (СН2) and methyl (СН3) protons of the mobile lipid domains (MLD) and choline, which are characteristic of apoptotic rather than of necrotic cells. A strong linear correlation between MLD content (calculated as CH2/CH3 signal intensity ratio) and the number of apoptotic cells in Namalwa or МТ4 cell lines has been shown for any inducer of apoptosis used in the study. MLD content estimated by 1H MRS technique correlated significantly with apoptotic cells numbers (r?=?0.992) recorded by conventional­ techniques. The increase in MLD content was regis­tered as early as 60 min after the addition of etoposide coinciding with the time course of caspase-3 activation.

Key words: proton nuclear magnetic resonance spectroscopy, flow cytometry, apoptosis, necrosis, mobile lipid domains, etoposide, fludarabine, quercetin, caspase-3.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal