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Journal archive > 2007 > N 3 May-June

COMPARATIVE INVESTIGATION OF CALIXARENES INFLUENCE ON Mg2+-DEPENDENT ATP
HYDROLASE ENZYMATIC SYSTEMS FROM SMOOTH MUSCLE CELLS OF THE UTERUS
R. D. Labyntseva1, N. М. Slinchenко1 , Т. О. Vекlіch1, R. V. Rodik2, S. О. Chеrеnок2,
V. I. Boiko2, V. I. Kalchenko2, S. О. Коstеrіn1
1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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2Institute of Organic Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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Investigation the influence of calyx[4]arenes C-90, C-91, C-97 and C-99 (codes are indicated) on the enzymatic activity of four functionally different Mg2+-dependent ATPases from smooth muscle of the uterus: actomyosin ATPase, transporting Ca2+,Mg2+-ATPase, ouabain-sensible Na+,K+-ATPase and basal Mg2+-ATPase. It was shown that calixarenes C-90 and C-91 in concentration 100 µM act multidirectionally on the functionally different Mg2+-dependent ATP-hydrolase enzymatic systems. These compounds activate effectively the actomyosin ATPase (Ка?=?52?±?11 µМ и 8 ± 2 µМ, accordingly), at the same time calixarene C-90 inhibited effectively activity of transporting Ca2+,Mg2+-ATPase of plasmatic membranes (I0,5 = 34,6 ± 6,4 µМ), but influence on membrane-bound Na+,K+-ATPase and basal Mg2+-ATPase. Calixarene C-91 reduce effectively basal Mg2+-ATPase activity, insignificantly activating Na+,K+-ATPase but has no influence on transporting Ca2+,Mg2+-ATPase activity of plasmatic membranes. Calixarenes C-97 and C-99 (100 µМ), which have similar structure, have monodirectional influence on activity of three functionally diffe­rent Mg2+-dependent ATPases of the myometrium: actomyosin ATPase and two ATPases, that related to the ATP-hydrolases of P type?– Ca2+,Mg2+-ATPase and Na+,K+-ATPase of plasmatic membranes. Basal Mg2+-ATPase is resistant to the action of these two connections. Results of comparative experiments that were obtained by catalytic titration of calixarenes C-97 and C-99 by actomyosin ATPase (I0,5 = 88 ± 9 and 86 ± 8 µМ accordingly) and Na+,K+-ATPase from plasmatic membranes (I0,5 = 33 ± 4 and 98?±?8?nM accordingly) indicate to the considerably more sensitiveness of Na+,K+-ATP-ase to these calixarenes than ATPase of contractile proteins. Thus, it is shown that calixarenes have influence on activity of a number of important enzymes, involved in functioning of the smooth muscle of the uterus and related to energy-supplies of the process of the muscle contracting and support of intracellular ionic homeostasis. The obtained results can be useful in further researches, directed at the use of calixarenes as pharmaceutical substance, able to normalize the contractile function of the uterus at some pregnancy pathologies in women’s.

Key words: actomyosin-ATPase, Са2+,Mg2+-ATPase, Na+,K+-ATPase, Mg2+-ATP­ase, plasma membrane, smooth muscle cells, myometrium, calixarene.

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