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Journal archive > 2007 > N 3 May-June

SECRETORY IgAs FROM HUMAN MILK WITH AFFINITY TO MAMMALIAN DNA
ARE CAPABLE OF HYDROLYZING RIBOSOMAL RNA

Yu. Ya. Kit1, E. V. Kuligina2, V. A. Richter2, R. S. Stoika1

1Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv;
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2Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Novosibirsk

It was found that milk of clinical healthy women contains sIgA possessing high affinity for the mammalian thymus DNA and DNA-hydrolyzing activity (sIgA-abzymes). Here we present data that such sIgA-abzymes, purified by sequential chromatography on DEAE-fractogel, heparin-sepharose, DNA-cellulose and followed by gel-filtration, are also able to hydrolyse total RNA from E. coli better than plasmid DNA. Besides, such sIgA-abzymes effectively cleaved 18S and 28S ribosomal RNA isolated from human A549 cells. It is noteworthy that the nuclease activity of sIgA-abzymes was significantly inhibited by ATP, while dATP had no effect on it. A potential role of the ribonuclease activity of sIgA-abzymes present in human milk is discussed.

Key words: abzymes, sIgA, human milk, nuclease activity, inhibitors.

The original article in English is available for download in PDF format.

© The Ukrainian Biochemical Journal