G. A. Becerril Aragon1,2, L. S. Starikovich1, A. Kolodkin2, І. V. Gorot3, М. М. Veliky4
Two fast migrating, major, multiple molecular forms (MMF) of glucose-6-phosphate dehydrogenаse [EC:188.8.131.52]: G-6-PDH-1 and G 6-PDH-2, and two minor forms: G-6-PDH-3 and G-6-PDH-4 were revealed in the electrophoregrams of both erythrocytes haemolisates as well in the homogenates of bone marrow cellular lines of rats at control conditions. Daily 1 cGy irradiation of rats up to a cumulative dose of 20 cGy led to a drop of G 6 PDH total activity and it caused a redistribution of the MMF of the enzyme in bone marrow cellular populations. However, G-6-PDH activity in erythrocytes exceeded the control means in all the experimental terms. The calculation of the local redistribution coefficient (lG-6-FDH-і) showed that these changes are mainly determined by the increase of the activity of the isoform G-6-PDH-3. Vitamin D3 administration to rats generated a correction of G-6-PDH activity in all studied cellular populations. Meanwhile, the MMF profiles were characterized by multidirectional rearrangements in the bone marrow erythroid and granulocyte-monocyte cells and in erythrocytes. The specificity of changes in the distribution of the MMF of G-6-PDH in the three studied cellular populations depends on the particularities of their energetic metabolism at irradiation conditions and on the modifying action of the natural adaptogen 1,25-dihydroxicholecalciferol.
Key words: enzyme, multiple molecular forms, activity, low dose radiation, vitamin D3, adaptogen, redistribution coefficient.
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