title en

Journal archive > 2007 > N 2 March-April 

V. V. Dmitrenko1, T. V. Bukreyeva1, K.?O.?Shostak1, N. Ya. Vitak1, Yu.?A.?Pekarsky1,
T. A. Malysheva2, M.?I.?Shamayev2, V. D. Rozumenko2, V.?V.?Kondratyuk2,
Yu. P. Zozulya2, V.?M.?Kavsan1
1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine, Kyiv

A comparison of gene expression profiles in different types of human brain tumours and normal brain by Serial Analysis of Gene Expression (SAGE) revealed exceptionally high content of CTTGGGTTTT tag in meningioma and ependi­moma SAGE-libraries. A search of the most rele­vant gene for this tag on the website «SAGE Anatomic Viewer» showed that it belonged to the nucleotide sequence of insulin-like growth factor II (IGF-ІІ) gene as well as to the open reading frame 43 on a chromosome 11 (C11orf43). This nucleotide sequence encodes putative insulin-like growth factor?II associated protein (IGF-ІІA). mRNA for this protein is produced as a result of the processing of IGF-ІІ gene primary transcript. Northern analysis of glial tumours and meningiomas showed the exceptionally high level of mRNA of IGF-ІІ-associa-ted protein in meningio­mas. Protein, encoded by this mRNA, can play the important role in menin-gioma formation and may be used as their specific molecular marker.

Key words: meningioma, ependimoma, serial analysis of gene expression (SAGE), IGF-ІІ, mRNA of IGF-ІІ-associated protein.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal