title en

Journal archive > 2007 > N 2 March-April 

DETERMINATION OF QUANTITY OF PHENOL COMPOUNDS USING
Pseudomonas FREE BACTERIAL CELLS

G. A. Gutnik, A. M. Zadorozhnyaya, T.?G.?Gruzina, V. V. Vember, D. A. Budankov, Z. R. Ulberg

Ovcharenko Institute of Biocolloidal Chemistry, National Academy of Sciences of Ukraine, Kyiv;

The possibility of using Pseudomonas free bacterial cells for integral evaluation of some phenol compounds’ content in water by means of estimation of specific respiratory activity (SRA) value was shown. Amperometric system of Clark electrode was used as a transducer in conducted investigations. The conditions of performing the SRA analysis of Pseudomonas sp. B 4251 and Ps. putida sp. microbial cells were optimized. The method sensitivity is 0.2 mM for Pseudomonas sp. B 4251 and 0.5 mM for Ps. putida sp. Taking into account specificity and sensitivity of the studied method, for estimating the integral toxicity of waste water, which contains 4-nitrophenol and 2,4-dinitrophenol in more than MPC concentrations and harmful for ecosystem became possible.

Key words: bacteria, specific respiratory activity, phenol compounds, biosensor.

The original article in Ukrainian is available for download in PDF format.

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