V. I. Lushchak1, P. Murray2, A. R. Cossins2
The increase of environmental temperature at physiological range can cause oxidative stress in exotherms, which in most cases leads to activation of the antioxidant enzyme, manganese-1 superoxide dismutase (Mn-SOD). This work is aimed to evaluate the changes in Mn-SOD enzyme activity and mRNA levels in the common carp, Cyprinus carpio, during transition from lower (15 °C) to higher (25 and 30 °C) temperatures. In liver, 25 °C exposure elicited little effect, but at 30 °C there was a significant increase in both Mn-SOD enzyme activity and mRNA levels. In brain enzyme activity was maximal at 25 °C and surprisingly, this increased activity was accompanied by a decrease in mRNA levels. This work suggests that the activity of Mn-SOD in carp is regulated by environmental conditions through transcriptional, translational and post-translational mechanisms, the particular mechanism used being dependent upon the tissue type.
Key words: common carp, Cyprinus carpio, temperature adaptation, antioxidant enzymes, manganese superoxide dismutase, catalase, mRNA, PCR.
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