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Journal archive > 2007 > N 2 March-April 

THE EFFECT OF TEMPERATURE INCREASE ON THE EXPRESSION
OF?MANGANESE?SUPEROXIDE DISMUTASE IN TISSUES
OF COMMON CARP?Cyprinus carpio

V. I. Lushchak1, P. Murray2, A. R. Cossins2

1Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine;
е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.;
2School of Biological Sciences, The University of Liverpool, Bioscience Centre, United Kingdom

The increase of environmental temperature at physiological range can cause oxidative stress in exotherms, which in most cases leads to activation of the antioxidant enzyme, manganese-1 superoxide dismutase (Mn-SOD). This work is aimed to evaluate the changes in Mn-SOD enzyme activity and mRNA levels in the common carp, Cyprinus carpio, during transition from lower (15 °C) to higher (25 and 30 °C) temperatures. In liver, 25 °C exposure elicited little effect, but at 30 °C there was a significant increase in both Mn-SOD enzyme activity and mRNA levels. In brain enzyme activity was maximal at 25 °C and surprisingly, this increased activi­ty was accompanied by a decrease in mRNA levels. This work suggests that the activity of Mn-SOD in carp is regulated by environmental conditions through transcriptional, translational and post-translational mechanisms, the particular mechanism used being dependent upon the tissue type.

Key words: common carp, Cyprinus carpio, temperature adaptation, antioxidant enzymes, manganese superoxide dismutase, catalase, mRNA, PCR.

The original article in English is available for download in PDF format.

© The Ukrainian Biochemical Journal