title en

Journal archive > 2007 > N 2 March-April 

IDENTIFICATION OF MECHANISM OF ENZYME/TRANSPORT PROCESS
(THE SYSTEM IS “ENZYME/TRANSPORTER – SUBSTRATE – EFFECTOR”)
USING THE “RATIO” METHOD

S. O. Karakhim, V. F. Gorchev, S. O. Kosterin

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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Using equilibrium assumption the analysis of kinetics of enzyme/transport process has been developed. In the course of this process the enzyme can interact simultaneously with substrate S and effector E and as a result two products are genera­ted: Р1 (from substrate) and Р2 (from effector) after catalytic effect of enzyme or transportation across the biological membrane. It has been demonstrated that the ratio (R = V0,1/V0,2) of initial rates of formation of reaction products Р1 (V0,1) and Р2 (V0,2?) represented in linearized form as a dependence on concentration of both substrate S0 and effector Е0 allows to identify a specific mechanism of the present process. An algorithm of such identification has been developed.

Key words: enzyme, transporter, substrate, effector, modification coefficients, Michaelis constant, limiting rate of enzyme-catalysed reaction, mechanism of enzyme-catalysed reaction, linearization criterion.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal