title en

Journal archive > 2007 > N 2 March-April 

ISOLATION OF SURFACE ANTIGEN OF HEPATITES B VIRUS

I. V. Nikolaenko1, V. S. Goncharenko1, N. N. Shimko1, A. Yu. Galkin2

1Joint Stock Company «Vitrotest», Kyiv, Ukraine;
National Technical University of Ukraine «Kyiv Polytechnical Institute»;

The scheme of immunoaffinity isolation of surface antigen of hepatitis B virus is developed. Selection of monoclonal antibodies was carried out and conditions for synthesis of immunoaffinity matrix were optimized; specificity of binding of HBs-antigen with a sorbent is improved; conditions of washing of columns and elution of HBs antigen are chosen. The yield of purified HBs-antigen obtained according to the developed scheme exceeded 90% and it allows to use such HBsAg for immunization of animals and application in immunoanalysis

Key words: HBsAg, purification of HBsAg­, immunoaffinity chromatography.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal