title en

Journal archive > 2007 > N 1 January-February

VISUALIZATION OF BACTERIOPHAGE T7 RNA-POLYMERASE COMPLEXES
WITH DNA TEMPLATE UNDER TRANSCRIPTION ELONGATION

A. P. Limanskii

Institute of Microbiology and Immunology, Academy of Medical Sciences of Ukraine, Kharkov;

Complexes of bacteriophage T7 RNA polymerase (T7 RNAP) with a DNA template (which contains a promoter and internal terminator of T7?RNAP) in transcription elongation were visualized by atomic force microscopy (AFM). Images of specific stable complexes of T7 RNAP with a terminal fragment of DNA template located at a distance of 200 bp from the T7 RNAP promoter were obtained for single molecules. Complexes of a single DNA template molecule with 2–3 T7 RNAP molecules corresponding to stages of initiation, elongation and termination of trans­cription were visualized under the elimination of nonspecific DNA-protein binding. Detailes of specific and nonspecific complex formation for the T7 RNAP?– DNA system during initiation and transcription elongation are discussed.

Key words: transcription, T7?RNA polymerase, promoter, atomic force microscopy.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal