A. P. Limanskii
Complexes of bacteriophage T7 RNA polymerase (T7 RNAP) with a DNA template (which contains a promoter and internal terminator of T7?RNAP) in transcription elongation were visualized by atomic force microscopy (AFM). Images of specific stable complexes of T7 RNAP with a terminal fragment of DNA template located at a distance of 200 bp from the T7 RNAP promoter were obtained for single molecules. Complexes of a single DNA template molecule with 2–3 T7 RNAP molecules corresponding to stages of initiation, elongation and termination of transcription were visualized under the elimination of nonspecific DNA-protein binding. Detailes of specific and nonspecific complex formation for the T7 RNAP?– DNA system during initiation and transcription elongation are discussed.
Key words: transcription, T7?RNA polymerase, promoter, atomic force microscopy.
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