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Journal archive > 2006 > N 4 July-August

APOPTOSIS OF HUMAN NAMALWA MALIGNANT LYMPHOID
CELLS INDUCED BY RESVERATROL AND QUERCETIN

А. А. Philchenkov1, М. P. Zavelevich1, N. N. Khranovskaya2, V. M. Mikhailenko1

1R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology,
National Academy of Sciences of Ukraine, Kyiv;
2Institute of Oncology, Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The effects of naturally-occuring polyphenols, resveratrol and quercetin, on cell viability and apoptosis were studied  in Namalwa B-cell lymphoma line. Apoptotic cells were identified using DNA flow cytometric analysis and 1H NMR spectroscopy. The effects of the agents on the cell cycle kinetics and activation of caspase-3 were exa­mined. Both resveratrol and quercetin induced apoptosis in Namalwa cells as demonstrated by the increased number of hypodiploid cells, elevated level of mobile lipid domains and caspase-3 activation. Treatment with 40 microM of resveratrol for 48 h resulted in time-dependent cell-cycle arrest at G0/G1. In contrast, upon quercetin treatment Namalwa cells accumulated in G2/M. Obtained results suggest that resveratrol and quercetin induced caspase-dependent apoptosis in human malignant lymphoid cells in vitro. These findings provide a rationale for further studies of in vivo effects of those polyphenols.

Key words: malignant lymphoid cells Namalwa, resveratrol, quercetin, apoptosis, mobile lipid domains, caspase-3, 1H NMR.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal