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Journal archive > 2006 > N 4 July-August


А. А. Philchenkov1, М. P. Zavelevich1, N. N. Khranovskaya2, V. M. Mikhailenko1

1R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology,
National Academy of Sciences of Ukraine, Kyiv;
2Institute of Oncology, Academy of Medical Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The effects of naturally-occuring polyphenols, resveratrol and quercetin, on cell viability and apoptosis were studied  in Namalwa B-cell lymphoma line. Apoptotic cells were identified using DNA flow cytometric analysis and 1H NMR spectroscopy. The effects of the agents on the cell cycle kinetics and activation of caspase-3 were exa­mined. Both resveratrol and quercetin induced apoptosis in Namalwa cells as demonstrated by the increased number of hypodiploid cells, elevated level of mobile lipid domains and caspase-3 activation. Treatment with 40 microM of resveratrol for 48 h resulted in time-dependent cell-cycle arrest at G0/G1. In contrast, upon quercetin treatment Namalwa cells accumulated in G2/M. Obtained results suggest that resveratrol and quercetin induced caspase-dependent apoptosis in human malignant lymphoid cells in vitro. These findings provide a rationale for further studies of in vivo effects of those polyphenols.

Key words: malignant lymphoid cells Namalwa, resveratrol, quercetin, apoptosis, mobile lipid domains, caspase-3, 1H NMR.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal