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Journal archive > 2006 > N 4 July-August

OXIDATIVE PROPERTIES OF PHOTOEXCITED FULLERENES C60
AND C60-CONTAINING COMPOSITES IN SUSPENSION OF THYMOCYTES
AND ERLICH ASCITES CARCINOMA CELLS SUSPENSION

S. V. Prylutska1, I. I. Grynyuk1, A. P. Burlaka2, O. А. Golub1, О. P. Маtyshevska1

1Taras Shevchenko Кyiv National University, Ukraine;
2Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine;

Biological effects of photoexcited in UV/VIS region water-soluble fullerenes С60 and synthesized С60-containing composites after its addition to cell incubation medium are studied. It is shown by EPR method, that in the presence of photoexcited С60 (10-5 М) reactive oxygen species (ROS) are generated both in water solution and cell suspensions. ROS generation is accelerated if С60 is included into aminopropylaerosyl or antracenaliminopropylaerosyl. Photodynamic damage of Erlich ascites carcinoma cells by photoexcited С60–composites is confirmed by a decrease of cell viability, enhanced content of Schiff base fluorescent products of malonaldehyde as indicator of lipid peroxidation and the decrease in respiratory activity, estimated by MTT-test. No effects of photoexcited С60–composites were detected in thymocytes suspension. The data obtained testity to perspective utility of С60–composites for optimization of photodynamic therapy methods.

Key words: ROS, EPR-analysis, MTT-test, lipid peroxidation, fullerene С60, С60–composite, thymocytes, Erlich ascites carcinoma cells.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal