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Journal archive > 2006 > N 5 September-October

IN Nicotiana tabacum L., INDUCED BY EXOGENIC DNA

V. A. Katsan, A. I. Potopalsky

Institute of Molecular Biology and Genetics,  National Academy of Sciences of Ukraine, Kyiv;
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The technology of obtaining the tobacco plants possessing the hereditary changes in the photosynthesis pigments accumulation during development using exogenous DNA has been elaborated. The plants possessing changes in proportion of chlorophylls content in the leave tissues, inherited elevated carotenoid content and altered proportion of carotene and xanthophylls, luteine and violaxanthine have been obtained by action of the salt-tolerant nightshade (Solanum nigrum L.) DNA and the DNAs of pCAMVNEO, pTi8628 plasmids on the tobacco cultivar Krupnolystny 20 (Large-leaf) germinating seeds. These plants have simultaneously the useful features – accelerated development, early blooming phenotype and higher productivity. Possible mechanisms emphasized such inherited biochemical changes have been discussed.

Key words: exogenous DNA, tobacco, chlorophylls, carotene, xanthophylls, violaxanthine, luteine, content ratio.

The original article in Ukrainian is available for download in PDF format.

© The Ukrainian Biochemical Journal