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Journal archive > 2006 > N 5 September-October

LECTINS MODULATE THE CYTOSTATIC ACTION OF THE COMPOSITE BIOREGULATORS CONJUGATES
OF AZAANALOGUES OF PYRIMIDINE AND PHENAZINE-1-CARBOXILIC ACID IN Bacillus subtilis TEST-SYSTEM

I. S. Karpova1, L. G. Palchikovska1, N. V. Koretska1, M. O. Platonov1, E. O. Kovalenko2, K. I. Getman2

1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv;
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

The idea of the work was to study a combined effect of some lectins (Con A, PHA, STA, WGA, SNA, VAA) and new composite bioregulators (PhCA-1 and azapyrimidine derivatives) on growth of Bacillus subtilis cells in order to elucidate cell targets sensitive to lectin’s activity. Study of combined effects of high- and low-molecular bioregulators may also be the subject of practical interest with a prospect of obtaining new bacterio- and carcinostatic drugs. Using B. subtilis mutants it was shown that lectins can modulate the cytostatic effect of initial substanses and their pyrimidine derivatives in the range: phenazine < PhCA-1 < 6-azacytosine derivative of PhCA-1 < 6-azauracyl derivative of PhCA-1. This modulating effect was absent in recP mutant, which has lost the ability to produce its own bacterial lectin, demonstrating its possib­le role as a mediator. The antagonistic effect of all plant lectins under study on cytostatic action of <6-azauracyl derivative of PhCA-1 in rec+ culture was observed as the result of possible competition for some common target. As both the B. subtilis lectin and the low-molecular bioregulator can inhibit transcription in vitro, it is supposed that their common target may be the DNA-dependent synthesis of RNA.

Key words: lectins, bioregulators, PhCA-1 and azapyrimidine derivatives, mutants of Bacillus subtilis, transcription in vitro.  

The original article in Ukrainian is available for download in PDF format.

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