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Journal archive > 2006 > N 5 September-October

REGULATION OF INTRACELLULAR LIPID METABOLISM BY THE PREPARATION OF ?-3 PHOSPHOLIPIDS
FROM MARINE ORGANISMS UNDER DEFICIT OF ESSENTIAL FATTY ACIDS IN RATS

P. V. Chernyavsky, Z. M. Datsenko, L. G. Moiseyeva, N. V. Kanivets, O. P. Kozulina, A. M. Boroda, O. S. Abakumova

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
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It has been established that a deficit of essential fatty acids (EFA) in the animal organism induces specific modifications of composition of fatty acid (FA) of general phospholipids and plasmalogenic Pl in microsomal tissue membranes with various functions and affects the activity of phospholipase A2. It has been shown that arachidonic (AA), docosapentaenoic (DPA) and docosahexaenoic (DHA) acids in the composition of general phospholipids – phosphatidylcholine (PC), phosphatidylethanolamine (PEA) and plasmalogens PC and PEA react to EFA deficit in the organism. Quantitative redistribution of AA, DPA, DHA of FA in general phospholipids and plasmalogenic microsomal membranes depending on their functions was found under EFA deficit in the organism. Deficit of DHA and plasmalogenic phospholipids evidences that the status of cell plasmalogens affects the level of PUFA at EFA deficit in the organism. AA and DHA can be a selective target for plasmalogens. The drug of ?-3 phospholipids, considerable amount of DHA and eicosapentaenoic (EPA)FA being present in their structure, increases the amount of plasmalogens and decreases the amount of AA in the brain, heart and reproductive organs. It was also found that EFA deficit in the organism favours the increase of lisoPEA, lisoPC, free FA (FFA) connected with the increase of activity of endogenic phospholipase A2 and plasmalogen-selective phospholipase A2. The ?-3 phospholipase from marine organisms at EFA deficit decreases the amount of FFA, lisophospholipids and activity of phospholipase A2 in the microsomas of studied tissues. The drug of ?-3 phospholipids normalizes the state and functions of the brain, liver and heart tissues, reproductive organs against a background of EFA deficit and regulates synthesis of biologically active metabolites of AA in the organism.

Key words: deficit of essential fatty acids, fatty acids of general phospholipids and plasmalogens, lisophospholipids, free fatty acids, phospholipase A2, plasmalogen-selective phospholipase A2, drug of ?-3 phospholipids from marine phospholipids.

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