Journal archive > 2006 > N 2, March-April


А. К. Gulevsky, Ye. А. Grishchenkova, L. I. Relina

Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkov;
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The level of antioxidant protection and lipid peroxidation (LP) intensity in the darkling beetle Tenebrio molitor on different developmental stages were assessed. Each stage was shown to be characterized by its own peculiarities of prooxidant-antioxidant balance. Thus, maximal intensity of oxidative processes estimated by LP intermediate product (diene conjugates and ketodiens) accumulation is attributable to pupae, and minimal intensity – to the 3rd–5th instar larvae. Superoxide dismutase activity increases gradually during the life cycle. A decline in catalase (CAT) and glutathione reductase (GR) activities occurred on the stage of pupae. CAT activity in imago was equal to the larva values, and GR activity in imago even exceeded the larva values. At the same time GR activity in T. molitor was detected only at 37 °С under our experimental conditions. No statistically significant changes in glutathione reduced content were observed in the insects during the life cycle.

Key words: lipid peroxidation, antioxidant enzymes, darkling beetle.

Recieved: 0000-00-00
Published at the site: 2006-09-14

The original article in Russian is available for download in PDF format.

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