ROLE OF CATALASE AND SUPEROXIDE DISMUTASE IN THE YEAST Saccharomyces cerevisiae RESPONSE TO HYDROGEN PEROXIDE IN EXPONENTIAL PHASE
Vassyl Stefanyk Precarpathian National University, Ukraine;
The role of catalase аnd superoxide dismutase (SOD) in response of the yeast Saccharomyces cerevisiae to oxidative stress induced by hydrogen peroxide in the middle-exponential phase has been investigated. It was shown that cell survival is significantly decreased after yeast exposure to hydrogen peroxide in the strains defective in cytosolic or peroxisomal catalases. Treatment of the wild-type сells with 0.5 mM Н2О2 for 30 min causes an increase in the activity of catalase and superoxide dismutase, but the effect was not observed in all strains investigated. It was also shown that hydrogen peroxide leads to an increase in the activities of both catalases and Cu,Zn-containing SOD. The effect was cancelled by cycloheximide, an inhibitor of protein synthesis.
Кey words: Saccharomyces cerevisiae, exponential phase, hydrogen peroxide, catalase, superoxide dismutase.
Published at the site: 2006-09-14
The original article in Ukrainian is available for download in PDF format.
© The Ukrainian Biochemical Journal