IDENTIFICATION OF CHANGES IN GENE LOCI POTENTIALLY ASSOCIATED WITH CERVICAL CANCER USING NotI MICROARRAYS
1Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv;
2Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden;
3State Research Centre of Virology and?Biotechnology VECTOR, Koltsovo, Russia;
4Institute of Oncology, Academy of?Medical Sciences of Ukraine, Kyiv;
5Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv
The investigation of the cancer-associated structural and epigenetic changes in cell genome is a major approach for understanding mechanisms of cancerogenesis. To investigate these genome changes, novel technique of microarrays comprising NotI-linking genome clones was developed. Twenty eight samples from patients with cervical cancer were analyzed using NotI microarrays of human chromosome 3. Deletions, amplifications and methylation were detected for 109 out of 182 NotI clones with different frequency. Notably, 17 NotI-linking clones showed genomic changes in more than 35% of tumor samples investigated, which suggests involvement of genes associated with these clones in development of cervical cancer.
Key words: microarrays, genomic DNA, differential gene expression, NotI clones, homozygous deletion, hemizygous deletion, DNA methylation, human chromosome 3, biological tumor markers.
Published at the site: 2006-09-18
The original article in Russian is available for download in PDF format.
© The Ukrainian Biochemical Journal