DETERMINATION OF Na+,K+-ATPase ACTIVITY IN HUMAN COLORECTAL CARCINOMA
1Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
2Taras Shevchenko National University, Kyiv;
3Institute of Oncology, Academy of Medical Sciences of Ukraine, Kyiv
Na+,K+-ATPase activities in macroscopically unchanged mucosa (conditionally normal tissue) and human colorectal carcinoma (mainly low-grade and moderately differentiated adenocarcinomas) have been investigated. Microsomal fractions are similar by dimensions of the membrane fragments detected by photon correlation spectroscopy analysis. The activation optima under digitonin pretreatment of the membrane fractions differ significantly for Na+,K+-ATPase and concomitant Мg2+-ATPase activity, but are the same in conditionally normal and cancerous tissues. This allows to detect correctly total levels of the Na+,K+-ATPase activity in the detergent-pretreated preparations. The moderate decrease of the Na+,K+-ATPase activity is revealed in carcinomas. It is concluded that a decrease of activity of the ouabain-sensitive human Na+,K+-ATPase is characteristic of colorectal carcinoma.
Key words: Na+,K+-ATPase, plasma membrane, colorectal carcinoma.
Published at the site: 2006-09-18
The original article in Russian is available for download in PDF format.
© The Ukrainian Biochemical Journal