OBTAINING OF BACTERIAL BIOLUMINESCENT STRAIN
Protobacterium phosphoreu В7071 (lux+) FOR ZINC IONS
Ovcharenko Institute of Biocolloid Chemistry, National Academy of Sciences of Ukraine, Kyiv;
The transconjugate containing hybrid plasmid (TeRZnRlux+) was obtained using the conjugation method on Pseudomonas fragi T2(5) (TeRZnR) strain and bioluminescent strain Protobacterium phosphoreum В7071 (lux+). The expression regulation of lux-genes on the obtained plasmid is carried out by promotor-operational area conjointly with zinc resistance genes.
The cells of the obtained genetically modified bacteria have the ability to specific induced luminescence, which is a respond to zinc ions’ presence in the measuring medium.
It was shown that the cells’ bioluminescence intensity of trans-conjugate is linearly dependent on zinc ions’ concentration within the range of 1–100 µM, that provides the opportunity of using biosensor as a strain for qualitative and quantitative detection of the metal.
The low sensitivity limit of this method is 0.5 µM for the metal. Besides having high sensitivity, the developed lux-biosensor is highly specified.
Key words: bacteria, conjugation, bioluminescence, zinc, biosensor.
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