Journal archive > 2006 > N 1 January-February

OBTAINING OF BACTERIAL BIOLUMINESCENT STRAIN
Protobacterium phosphoreu В7071 (lux+) FOR ZINC IONS
CONCENTRATION DETECTION

T. G. Gruzina, S. N. Dibkova, T. P. Chekhovskaya, V. V. Vember, А. М. Zadorozhnyaya, Z. R. Ulberg

Ovcharenko Institute of Biocolloid Chemistry, National Academy of Sciences of Ukraine, Kyiv;
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The transconjugate containing hybrid plasmid (TeRZnRlux+) was obtained using the conjugation method on Pseudomonas fragi T2(5) (TeRZnR) strain and bioluminescent strain Protobacterium phosphoreum В7071 (lux+). The expression regulation of lux-genes on the obtained plasmid is carried out by promotor-operational area conjointly with zinc resistance genes.
The cells of the obtained genetically modified bacteria have the ability to specific induced luminescence, which is a respond to zinc ions’ presen­ce in the measuring medium.
It was shown that the cells’ bioluminescence intensity of trans-conjugate is linearly dependent on zinc ions’ concentration within the range of 1–100 µM, that provides the opportunity of using  biosensor as a strain for qualitative and quantitative detection of the metal.
The low sensitivity limit of this method is 0.5 µM for the metal. Besides having high sensitivi­ty, the developed lux-biosensor is highly specified.

Key words: bacteria, conjugation, bioluminescence, zinc, biosensor.

The original article in Russian is available for download in PDF format.

© The Ukrainian Biochemical Journal