COMPARATIVE CHARACTERISTIC OF ACTIVITY OF UREASE,
INCORPORATED INTO POLYSILOXANE HYDROGELS AND XEROGELS,
OBTAINED BY SOL-GEL TECHNIQUE
Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kyiv;
Polysiloxane hydrogels with incorporated urease (degree of immobilization is 79–88%) that retains fermentative activity at the level of 56–84% were obtained by sol-gel technique. An influence of polysiloxane matrix functionalization on a degree of incorporation, activity retention and a factor of efficiency of urease immobilization was studied. Polysiloxane matrix functionalization with methyl groups causes decreasing a degree of ferment immobilization and a factor of immobilization efficiency. Functionalization of polysiloxane matrix with 3-aminopropyl groups leads to practically quantitative incorporation of the enzyme. And the highest degree of urease activity retention and maximal factor of its immobilization were observed at 3-aminopropyl groups content in polysiloxane matrix equals 2–5% (mol.). Transformation of hydrogels into xerogels via vacuum drying causes decreasing urease fermentative activity on nearly 2 orders.
Key words: sol-gel technique, hydrogel, xerogel, urease, immobilization, activity.
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