Journal archive > 2005 > N 4, July-August

Protein splicing

P. L. Starokadomskij

Inteins are internal polypeptide sequences that are posttranslationally excised from a protein precursor by a self-catalyzed protein-splicing reaction. Most of inteins consist of N- and C-terminal protein splicing domain and central endonuclease domain. The endonuclease domain can initiate mobility of the intein gene, this process being named intein homing. This review is focused on the recent data about the structure and function of inteins. Main intein-mediated protein-engineering applications, such as protein purification, ligation and cyclization, new forms of biosensors, are presented.

Recieved: 2005-03-18

Published at the site: 2005-07-04

The original article in Russian is available for download in PDF format.

 

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