Purification and study of carbohydrate specificity of lectin from Sarcoscypha coccinea (FR.) Lambette
A lectin that revealed affinity for lactose, N-acethylactosamine, 4-nitrophenyl-?-D-gluco- and galactopyranosides from fruiting bodies of Sarcoscypha coccinea (Fr.) Lambette was purified by affinity chromatography on immobilized ovomucoid.
According to electrophoresis data in 15% SDS-PAGE the lectin contains two very low-differing components with molecular weight 32 kDa. Molecular weight of the lectin is 128 kDa according to gel-chromatography on sephadex G-200. The lectin agglutinates rabbit erythrocytes and slightly weaker agglutinates human erythrocytes. After dialysis against 1% EDTA sodium salt solution the lectin loses hemaglutinating activity, but after the next dialysis against СаСl2 solution it is restored.
Published at the site: 2005-05-05
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